Thermo Scientific™ Acclaim™ HILIC-10 HPLC Columns

Use this HILIC column to retain and separate highly polar molecules that are unretained by reversed-phase chromatography.

Catalog Number	Mfr. No.	Length (Metric)	Diameter (Metric)	Particle Size
DX074259	Thermo Scientific™ 074259	150 mm	2.1 mm	3 μm
DX074263	Thermo Scientific™ 074263	10 mm	2.1 mm	5 μm

Description

Separate highly hydrophilic molecules by hydrophilic interaction liquid chromatography using Thermo Scientific™ Acclaim™ HILIC-10 LC Columns. These columns retain and separate highly polar molecules that are unretained by reversed-phase chromatography. Their high-purity, spherical, porous silica base is covalently modified with a proprietary hydrophilic layer. This chemistry enables the use of 5–20% aqueous mobile phase while maintaining affinity for polar analytes. Sample throughput is increased without the need to evaporate and reconstitute samples dissolved in nonaqueous solvents.

Rugged and Reproducible Separations of Highly Polar Compounds:

• Unique selectivity, complementary to reversed-phase columns
• Enhanced sensitivity for MS detection
• Hydrolytically stable
• Rugged column packing
• Broad application range, including separation of hydrophilic drugs and drug metabolites

Acclaim HILIC-10 LC columns are available in 3μm particle size and a variety of column formats: 4.6×150mm, for high-throughput routine analysis; 3.0×150mm, for high-throughput analysis with reduced solvent consumption; and 2.1×150mm, for LC-MS analysis.

Specifications

• For Use With: LC/MS
• Pore Size: 120 Å
• Temperature: 70°C
• Product Line: Acclaim
• Column Type: HILIC
• Phase: HILIC

• pH: 2 to 8
• Surface Area: 300 m²/g
• Stationary Phase: Proprietary
• Carbon Load: 8%
• Endcapped: Yes

Limited warranty period: Subject to all warranty requirements and exclusions set out in our Terms and Conditions of Sale, this product is warranted from the date we ship the product and for ninety (90) days thereafter. Disclaimer to internal research use restriction: Any restrictions on the purchaser to utilize the product(s) for internal research purposes only does not apply to the product(s) on the web pages associated with this statement.