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Gibco™ ExpiFectamine™ CHO Transfection Kit
The ExpiFectamine™ CHO Transfection Kit is a core component of the ExpiCHO™ Expression System Kit, designed for transient transfection of high-density cultures of Chinese hamster ovary (CHO) cells.
$1066.00 - $39770.00
Specifications
| Cell Line | ExpiCHO-S™, CHO-S, SFM Adapted |
|---|---|
| Classification | Animal Origin-free, Chemically-defined, Protein-free, Serum-free |
| Cell Type | Ovarian Cells |
| Transfection Technique | Lipid-based Transfection |
| Product Line | ExpiFectamine™ |
| Catalog Number | Mfr. No. | Quantity | Price | Quantity & Availability | |||||
|---|---|---|---|---|---|---|---|---|---|
| Catalog Number | Mfr. No. | Quantity | Price | Quantity & Availability | |||||
A29129
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Gibco™
A29129 |
For 1 L of culture |
Each for $1,066.00
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A29130
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Gibco™
A29130 |
For 10 L of culture |
Each for $9,080.00
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A29131
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Gibco™
A29131 |
For 50 L of culture |
Each for $39,770.00
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Description
The ExpiFectamine CHO Transfection Kit is a core component of the ExpiCHO Expression System Kit (Cat. No. A29133) and enables transient transfection of high-density cultures of Chinese hamster ovary (CHO) cells. The high-efficiency, cationic transfection reagent, coupled with an optimized transfection enhancer and culture feed, will help power the highest possible level of protein expression from ExpiCHO-S Cells (Cat. No. A29127) cultured in ExpiCHO Expression Medium (Cat. No. A2910001).
All components work together synergistically with expression yields of 1–3 g per L of transfected culture demonstrated for some antibody and non-antibody proteins.
Features of the ExpiFectamine CHO Transfection Kit include:
- Enables robust and reproducible transfection results, giving you greater confidence in your results and ability to use CHO cells from research to discovery
- Achieve >90% transfection efficiency of high-density suspension CHO cell culture, with matching transfection enhancer and culture feed to boost transfection performance and protein expression
- Uses one-third to one-half less plasmid DNA than other transfection reagents
- Scale growth and transfection in culture formats from <1 mL in deep well plates up to 5 L when using Nalgene angled-bottom shake flasks
- Flexible, reliable culture and transfection protocols with multiple options that readily fit into current transient expression workflows
The ExpiFectamine CHO Transfection Kit is specifically designed to work with ExpiCHO-S Cells in the ExpiCHO Expression System Kit. The transfection kit supplies the cationic ExpiFectamine CHO Transfection Reagent, ExpiFectamine CHO Transfection Enhancer, and ExpiCHO Feed. ExpiCHO-S Cells and ExpiCHO Expression Medium are available separately.
Specifications
| ExpiCHO-S™, CHO-S, SFM Adapted | |
| Ovarian Cells | |
| ExpiFectamine™ | |
| Room Temperature |
| Animal Origin-free, Chemically-defined, Protein-free, Serum-free | |
| Lipid-based Transfection | |
| CHO Transfection Kit |
For Research Use Only. Not for use in diagnostic procedures.
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