Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP, Invitrogen™

Goat Polyclonal Secondary Antibody

Supplier:  Thermo Scientific 32460

Catalog No. PI32460

Description

This antibody may cross-react with immunoglobulins from other species. Working dilutions for Western blotting using SuperSignal West Substrates: SuperSignal West Pico Substrate - 1:250-1:1250 SuperSignal West Dura Substrate - 1:600-1:3000 SuperSignal West Femto Substrate - 1:1250-1:6000 ECL Western Blotting Substrate - 1:20-1:125

Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

• Antigen: Rabbit IgG (H+L)
• Classification: Polyclonal
• Conjugate: HRP
• Host Species: Goat
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Target Species: Rabbit
• Form: Liquid

• Applications: ELISA, Immunohistochemistry, Western Blot
• Concentration: 0.01 mg/mL
• Formulation: PBS with proprietary stabilizer and no preservative
• Immunogen: Purified Rabbit IgG, whole molecule
• Quantity: 2 mL
• Primary or Secondary: Secondary
• Content And Storage: 4° C
• Isotype: IgG