Thermo Scientific Chemicals Guanidine hydrochloride, Molecular Biology Grade

Description

A protein denaturant

Guanidine hydrochloride is used for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. Guanidine hydrochloride is used in RNA isolation to dissociate nucleoproteins and inhibit RNase.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
Guanidine hydrochloride is used for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. Guanidine hydrochloride is used in RNA isolation to dissociate nucleoproteins and inhibit RNase.

Solubility
Freely soluble in water

Notes
Protect from heat. Store away from oxidizing agents.

Specifications

Chemical Identifiers

• CAS: 50-01-1
• Molecular Formula: CH6ClN3
• Molecular Weight (g/mol): 95.53
• MDL Number: MFCD00013026
• InChI Key: PJJJBBJSCAKJQF-UHFFFAOYSA-N
• Synonym: guanidine hydrochloride, guanidinium chloride, guanidine, monohydrochloride, aminoformamidine hydrochloride, aminomethanamidine hydrochloride, guanidine monohydrochloride, guanidine hcl, guanidine chloride, iminourea hydrochloride, carbamidine hydrochloride
• PubChem CID: 5742
• ChEBI: CHEBI:32735
• IUPAC Name: guanidine;hydrochloride
• SMILES: C(=N)(N)N.Cl

• Melting Point: 178°C to 185°C
• Color: White
• Density: 1.34
• Quantity: 100 g
• Beilstein: 3591990
• Sensitivity: Hygroscopic
• Merck Index: 144562
• Solubility Information: Freely soluble in water
• Formula Weight: 95.53
• Grade: Molecular Biology
• Physical Form: Crystalline Solid
• Chemical Name or Material: Guanidine hydrochloride

Safety and Handling

Hazard Category	Serious eye damage/eye irritation (category 2), Skin corrosion/irritation (category 2), Acute toxicity (category 4)
Hazard Statement	Causes serious eye irritation, May form combustible dust concentrations in air, Causes skin irritation, Harmful if swallowed or if inhaled
EINECSNumber	200-002-3
RTECSNumber	MF4300000
TSCA	Yes
Recommended Storage	Ambient temperatures

SDS

RUO – Research Use Only