Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Invitrogen™ Lipofectamine™ 3000 Transfection Reagent
GREENER_CHOICE

Lipofectamine™ 3000 Transfection Reagent leverages our most advanced lipid nanoparticle technology to provide superior transfection performance with improved application outcomes and reproducible results.

Supplier:  Invitrogen™ L3000015

Encompass_Preferred

 View more versions of this product

Catalog No. L3000015


Explore available promotions
Add to Cart

Description

Description

Lipofectamine™ 3000 Transfection Reagent leverages our most advanced lipid nanoparticle technology to provide superior transfection performance with improved application outcomes and reproducible results.

This reagent delivers superior transfection efficiency and improved cell viability for the widest range of hard-to-transfect and common cells (e.g., HEK293, HeLa).

Successfully transfect the widest variety of biologically relevant cell types with a reagent that offers:
  • Superior performance—our highest efficiency reagent for difficult-to-transfect cells
  • Improved cell viability—gentle on your cells, with low toxicity
  • Versatile—one reagent for DNA, RNA, and co-transfection

Efficiently Transfect Difficult-to-Transfect Cells
Lipofectamine 3000 reagent is designed to efficiently transfect difficult-to-transfect cells, yielding superior transfection performance across the broadest array of cell types. Successfully transfected cell types include:

Fibroblast (3T3, COS-7)
Myoblast (C2C12, L6 CRL-1458)
Hepatocyte (HepG2, HuH7)
Erythroleukemic cell (K562)
Breast carcinoma (MCF7, Hs578T)
Prostate carcinoma (LNCap)
Lung carcinoma (A549, NCI-H460)
Osteosarcoma (U-2 OS, Saos-2)
Colon carcinoma (Caco2, SW480)
Pancreatic carcinoma (PANC-1)
Skin melanoma (SK-MEL-28)

Transfect Common Cells Efficiently and Cost-Effectively
For common cell types, Lipofectamine 3000 reagent is more cost-effective than other reagents due to its high concentration and low usage amount. The 1.5mL size provides up to 1500 transfection reactions (in 24-well plates).

Achieve Superior Transfection Efficiency While Maintaining Low Toxicity
Lipofectamine 3000 reagent maintains a high transfection efficiency within a robust dynamic range of lipid doses for quick and easy optimization. A low toxicity lipid dose (0.75μL for 24-well plates) is suggested for applications requiring minimal disruption of the cells.

Enable High Transfection Efficiency in Novel Genome Editing Applications
Lipofectamine 3000 reagent is suitable for novel genome editing applications. It increases the likelihood of successful cleavage and recombination with TALENs or CRISPRs through highly efficient transfection, ultimately maximizing the efficiency of genetic modifications and simplifying the downstream processes.
TRUSTED_SUSTAINABILITY
Specifications

Specifications

Animal Origin-Free
Established Cell Lines, Stem Cells, Primary Cells, Hard-to-Transfect Cells
Plasmid DNA, Synthetic siRNA, RNAi Plasmids (shRNA, miR)
Transfection
Lipofectamine™
1.5 mL
Contains one vial of Lipofectamine™ 3000 Reagent (1.5 mL) and one vial of P3000TM Enhancer Reagent (1.5 mL) for 1000-1500 reactions in 24-well plates, or 200-250 reactions in 6-well plates. Store at 2–8°C. Do not freeze.
6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
Lipid-based Transfection
Sustainable packaging
Transfection Reagent
Yes
Product Suggestions

Product Suggestions

Safety and Handling

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov
SDS
Documents

Documents

Product Certifications
Promotions

Promotions

Product Content Correction

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

For Research Use Only. Not for use in diagnostic procedures.