Thermo Scientific™ L-Leucine for SILAC

Study protein expression by mass spectrometry with mass variants of lysine, arginine and other amino acids to perform stable isotope labeling with amino acids in cell culture.

Supplier:  Thermo Scientific™ 88428

Catalog No. PI88428

Description

Thermo Scientific™ Heavy and Light Amino Acids are used to specifically analyze protein expression by mass spectrometry using stable isotope labeling with amino acids in cell culture (SILAC) quantification kits.

SILAC is a powerful method to identify and quantify relative differential changes in complex protein samples. This approach entails the in vivo metabolic incorporation of “heavy“ ¹³C- or ¹⁵N-labeled amino acids into proteins followed by mass spectrometry (MS) for the accelerated and comprehensive identification, characterization and quantitation of proteins.

Highlights:

• Efficient – 100% label incorporation into proteins of living cells

• Flexible – different isotopes of heavy and/or light amino acids for arginine, lysine, leucine and proline enable the quantitation of peptides derived from MS-grade proteases

• Multiplex capabilities – several alternative isotopes of arginine and lysine are available that allow the analysis of multiple treatment conditions in each experiment

• High-quality supplements – heavy amino acids with >99% isotope purity

Specifications

• Description: L-Leucine
• Product Type: Amino Acids
• Label or Dye: Mass Isotope of Amino Acid
• Starting Material: Cells
• Detection Method: Mass Spectrometry
• Quantity: 500 mg

• For Use With (Equipment): Mass Spectrometer
• Content And Storage: Store at room temperature.
• Shipping Condition: Room Temperature
• Final Product Type: Protein
• Workflow Step: Protein Labeling
• Form: Solid

For Research Use Only. Not for use in diagnostic procedures.