Thermo Scientific™ Pierce™ Protein-Free Blocking Buffer

Pierce Protein-Free Blocking Buffer is a non-protein compound that provides effective blocking for membrane-based and plate-based protein detection methods, resulting in extremely low background.

Catalog Number	Mfr. No.	Description
PI37584	Thermo Scientific™ 37584	Protein-Free (PBS) Blocking Buffer, 100 mL
PI37572	Thermo Scientific™ 37572	Protein-Free (PBS) Blocking Buffer, 1 L
PI37585	Thermo Scientific™ 37585	Protein-Free (TBS) Blocking Buffer, 100 mL
PI37570	Thermo Scientific™ 37570	Protein-Free (TBS) Blocking Buffer, 1 L
PI37573	Thermo Scientific™ 37573	Protein-Free T20 (PBS) Blocking Buffer, 1 L
PI37571	Thermo Scientific™ 37571	Protein-Free T20 (TBS) Blocking Buffer, 1 L

Description

Pierce Protein-Free Blocking Buffer is a non-protein compound that provides effective blocking for membrane-based and plate-based protein detection methods, resulting in extremely low background. It is supplied in a convenient, ready-to-use format.

Features of Protein-Free Blocking Buffer include:
• Protein-free blocker—minimizes or eliminates cross-reactivity associated with protein-based blocking buffers
• Applications—compatible with western blotting (membranes), ELISA (microplates), and arrays (coated glass slides)
• Streptavidin-friendly—free of biotin; no interference with avidin-biotin detection systems
• High-performance—optimized and validated in many protein methods to provide high signal-to-noise ratio (i.e., no quenching of specific binding and signal but eliminating nonspecific binding and background)

Traditional blocking buffers contain proteins that can cross-react in certain immunodetection systems, resulting in high background and reduced signal. Pierce Protein-Free Blocking Buffers are devoid of protein while remaining highly effective at blocking plates, membranes, and other surfaces for ELISA, western blotting, glass slide arrays, and other applications.

Specifications

• Product Type: Blocking Buffer, protein-free
• Concentration: 1X
• Content And Storage: Upon receipt store product at room temperature. After opening, store product at 4°C.

• Form: Liquid
• Blocking Agent: Non-protein compound