Thermo Scientific™ Pierce™ Fast Western Kit, SuperSignal™ West Pico, Rabbit

For picogram-level detection and Western blot development less than 1 hour after gel transfer.

Supplier:  Thermo Scientific™ 35066

Catalog No. PI35066

Description

Thermo Scientific™ Pierce Fast Western Blot Kit, SuperSignal West Pico Substrate contains optimized reagents and protocol that allows picogram levels of detection and shortens typical Western blot development to less than 1 hour after the gel transfer.

These Fast Western Blot Kits require minimal hands-on time and yield results comparable to classic Western blotting with SuperSignal West Pico Chemiluminescent Substrate.The kits contain all the reagents necessary to complete a Western blot being probed with either a mouse or rabbit primary antibody. Like other Western blotting methods, the included SuperSignal West Pico Substrate produces a chemiluminescent signal that is detectable using photographic or other imaging methods. The developed blots can be repeatedly exposed and stripped of the primary antibody and reprobed.

Highlights:

• Fast – picogram levels of detection in about one hour

• Convenient – no expensive hardware or vacuum required; no clogging issues

• Simple – optimized protocol makes Western blot analysis easier than ever

• Economical – no additional consumables or extra equipment to purchase

• Stable – all kit components are stable for one year stored at 4°C

• Easy – complete kits contain all components needed to block, probe and develop a blot with any standard mouse or rabbit primary antibody

Compatible with:

Pierce Membranes and Filter Paper; CL-Xposure™ Film for Western blotting; Pierce Western Blot Signal Enhancer; Restore Plus™ Western Blot Stripping Buffer

Specifications

• Description: Pierce Fast Western Blot Kit, SuperSignal West Pico Substrate, Rabbit Trial Size
• Detection Method: Chemiluminescence
• For Use With (Application): Western Blot
• Product Line: Pierce™, SuperSignal™
• Conjugate: HRP
• Reactivity: Rabbit
• Sufficient For: 5 Mini Blots

• Product Type: Western Blot Kit
• Form: Liquid
• Membrane Compatibility: Nitrocellulose, PVDF
• Quantity: 200 mL
• Substrate: Horseradish Peroxidase
• Sensitivity: Picogram-level
• Content And Storage: Sufficient For: 5 mini blots probed with rabbit primary antibodies
  • Fast Western Antibody Diluent, 100 mL
  • Fast Western 10X Wash Buffer, 50 mL
  • Fast Western Rabbit Optimized HRP Reagent, Pico, 5 mL
  • SuperSignal West Pico Luminol/Enhancer Solution, 25 mL
  • SuperSignal West Stable Peroxide Solution, 25 mL
  
  Store kit at 4°C.

For Research Use Only. Not for use in diagnostic procedures.