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Molecular Probes™ Streptavidin, Alexa Fluor™ 633 conjugate
Streptavidin, Alexa Fluor™ 633 conjugate
Supplier: Molecular Probes™ S21375
Description
Alexa Fluor™ 633 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor™ dye). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays. Alexa Fluor™ dye streptavidin conjugates are supplied as 1 mg lyophilized product or in 0.5 mL volumes of a 2 mg/mL solution.Important Features of Alexa Fluor™ 633 Streptavidin Conjugates:
• Alexa Fluor™ 633 streptavidin conjugate has Ex/Em maxima of ∼ (632/647)
• Bright, photostable fluorescence
• High solubility in aqueous solutions
• Available in multiple colors
• Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more
Properties of Alexa Fluor™ Dyes
Alexa Fluor™ dyes are organic fluorescent dyes developed for better performance in imaging and other labeling protocols and exhibit improved photostability and brightness and improved solubility in aqueous solutions. Available in a broad range of colors, these dyes are a good choice for most imaging applications.
Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.
Specifications
| Alexa Fluor 633 | |
| Store in freezer (-5 to -30°C) and protect from light. | |
| Solid | |
| Streptavidin Conjugate (fluorescent) |
| 1 mg | |
| Room Temperature | |
| Alexa Fluor™ | |
| Alexa Fluor Dyes |
Safety and Handling
WARNING: Cancer - www.P65Warnings.ca.gov
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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