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Edvotek™ Mini-Prep Isolation of Plasmid DNA
Introduces the principles of extracting plasmid DNA from bacterial cells.
Supplier: Edvotek™ 202
Catalog No. S05638
Includes:
- Instructions
- Plasmid LyphoCells™
- Tris Buffer Concentrate
- Sodium Hydroxide Solution
- SDS Solution
- Resuspension Buffer
- Potassium Acetate Solution
- RNase Solution
- UltraSpec-Agarose™
- 10X Gel Loading Solution
- 50X Concentrated Electrophoresis Buffer
- Practice Gel Loading Solution
- FlashBlue™ DNA Stain
- InstaStain™ Blue Cards
- Disposable Pipets
- Microcentrifuge Tubes.
Requires :
- Electrophoresis tank
- Power supply
- Variable micropipet and tips
- Balance
- Microwave or hotplate
Description
Small-scale rapid isolation of plasmid DNA is a routine procedure used for screening and analysis of recombinant DNA in cloning and subcloning experiments.
- In this experiment, students isolate plasmid DNA without the use of toxic chemicals like phenol or chloroform
- Complete in 1 hour 45 minutes
Order Info
For 20 plasmid isolations and seven 7x7cm gels.
Specifications
| Agarose Gel electrophoresis | |
| Use for screening and analysis of recombinant DNA in cloning and subcloning experiments | |
| Instructions, plasmid extraction LyphoCells™, Gel-loading solution, UltraSpec-Agarose™ Powder, electrophoresis Buffer, InstaStain™ FlashBlue™ Stain, FlashBlue Plus™ |
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