Test
The MagMAX DNA Multi-Sample Ultra 2.0 Kit is an automatable magnetic bead-based sample preparation technology for the isolation of genomic DNA (gDNA) from 50 μL to 2 mL of whole blood, saliva, buffy coat, and buccal swabs.
Optimize cloning with Thermo Scientific™ pBR322 DNA, a 4361 bp double-stranded closed circular medium copy plasmid DNA.
GeneArt Gibson Assembly HiFi Master Mix enables DNA assembly and cloning via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a one-step, 15–60 minute isothermal reaction.
Shipping Condition | Dry Ice |
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Content And Storage | Includes master mix, positive control, and water and accommodates the use of your own competent cells. |
Workflow Step | DNA Assembly |
Number of Fragments | Up to 6 fragments |
Product Type | HiFi Master Mix |
Control Type | Positive Control |
Cloning Method | Seamless cloning |
For Use With (Application) | Seamless Cloning & Genetic Assembly |
Product Line | GENEART™ |
Enables the simultaneous and directional cloning of 1 to 4 PCR fragments, consisting of any sequence, into any linearized vector, in a single 30-minute room temperature reaction
The BigDye™ Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, plasmid, fosmid, and BAC templates.
Format | 12-well Strips,8-well Strip,96- or 384-well Plate |
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Product Type | Sequencing Buffer |
For Use With (Equipment) | SeqStudio™ Genetic Analyzer, SeqStudio™ Flex Series Genetic Analyzer, 3500/3500xL Genetic Analyzer, 3730/3730xl DNA Analyzer Thermal Cyclers: GeneAmp™ PCR System 9700 Dual Series, Veriti™ Fast 96‐well and 384‐well Thermal Cyclers |
Technique | Fluorescent Dye Terminator Sequencing |
Product Line | BigDye™ Terminator |
Generate DNA size marker fragments with this linear, double-stranded DNA, a common substrate for restriction endonucleases.
Provides quick, one-step cloning strategy for directly inserting Taq-amplified PCR product into plasmid vector
Promoter | T7 |
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Content And Storage | TA Cloning™ kits contain linearized pCR™2.1 vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls. Store all components at -20°C. All reagents are guaranteed stable for 6 months when properly stored. |
Format | Kit |
Product Type | Cloning Kit |
Cloning Method | TA Cloning |
Bacterial or Yeast Strain | Not Included |
For Use With (Application) | PCR Cloning |
Vector | pCR2.1 |
Product Line | TA Cloning™ |
Enable fast sticky-end or blunt-end DNA ligation in only 5 minutes at room temperature.
Product Type | Rapid DNA Ligation Kit |
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Enzyme | DNA Ligase |
Efficiently clone PCR products generated with any thermostable DNA polymerase using Thermo Scientific™ CloneJET™ PCR Cloning Kit, a positive selection system.
Antibiotic Resistance Bacterial | Ampicillin (AmpR) |
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Format | Kit |
Sample Type | PCR Products |
Cloning Method | Restriction Enzyme⁄ MCS, Blunt PCR |
For Use With (Application) | PCR Cloning |
Vector | pJET1.2/blunt |
Product Line | CloneJET |
Invitrogen™ RNase T1, RNA-Grade cleaves 3' of single-stranded G residues and is tested for purity to ensure that no contaminating ribonuclease activities are present that could cleave at unanticipated sites.
Optimize cloning with this double-stranded closed circular high copy plasmid DNA, 2686 base pairs with a molecular weight of 1.74 x 106 Da isolated from E.coli.
Optimize cloning with this double-stranded closed circular high copy plasmid DNA, 2686 base pairs with a molecular weight of 1.74 x 106 Da isolated from E.coli.
Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of blunt-ended PCR products amplified with proofreading thermostable polymerases into a plasmid vector
Use this size standard for fragments between 20 and 600 bp. The standard contains 36 LIZ dye-labeled, single-stranded DNA fragments.
Perform blunting and phosphorylation of DNA ends in just 5 minutes for subsequent use in blunt-end ligation with this kit.