Direct PCR Reagents and Kits

In hot start PCR, key reagents are prevented from reacting in the PCR mixture until optimal temperatures are met. This reduces non-specific DNA amplification that occurs naturally at lower temperatures, increasing product yield, specificity, and sensitivity.

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Invitrogen™ Platinum™ Direct PCR Universal Master Mix

Invitrogen Platinum Direct PCR Universal Master Mix is designed to amplify DNA directly from various samples without the need to purify DNA.

Promotions are available

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
Polymerase	Platinum II Taq Hot-Start DNA Polymerase
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	1X
Size (Final Product)	8 kb
Reaction Format	SuperMix or Master Mix
Product Type	Direct PCR Universal Master Mix
Reaction Speed	Fast
Hot Start	Built-In Hot Start
Overhang	3'-A
Green Features	Less hazardous, less waste
Concentration	2X
For Use With (Application)	Hot-start PCR,Real Time PCR (qPCR)

Thermo Scientific™ Phire Plant Direct PCR Master Mix

Thermo Scientific Phire Plant Direct PCR Master Mix is designed to amplify DNA directly from plant samples.

Pricing & Availability
Specifications

Shipping Condition	Wet or Dry Ice
Polymerase	Phire Hot Start II DNA Polymerase
GC-Rich PCR Performance	Medium
Reaction Format	SuperMix or Master Mix
Product Type	Direct PCR Master Mix
Sample Type	Plant
Reaction Speed	Fast
Hot Start	Built-In Hot Start
Overhang	Blunt
Concentration	2X
Starting Material	DNA
For Use With (Application)	Genotyping,Hot-start PCR
PCR Method	Direct PCR

Thermo Scientific™ GeneJET PCR Purification Kit PROMO

GeneJET PCR Purification Kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions.

Promotions are available

Pricing & Availability
Specifications

Format	Spin Column
Run Time	5 min.
High-throughput Compatibility	Not High-throughput Compatible (Manual)

Invitrogen™ PureLink™ PCR Purification Kit

The PureLink PCR Purification Kit provides a more convenient, rapid, and high-yielding purification process compared to other companies' kits.

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Format	Kit
Sample Type	PCR Products
Isolation Technology	Silica Spin Column
Run Time	10 min.

Thermo Scientific™ Phire Plant Direct PCR Kit (without sampling tools)

Thermo Scientific Phire Plant Direct PCR Kit is designed to amplify DNA directly from plant samples. No DNA purification is required prior to PCR.

Pricing & Availability

Thermo Scientific™ Phire Tissue Direct PCR Master Mix PROMO

Amplify DNA directly from variety of human and animal samples with no prior purification with Phire Tissue Direct PCR Master Mix.

Promotions are available

Pricing & Availability
Specifications

Polymerase	Phire Hot Start II DNA polymerase
GC-Rich PCR Performance	Medium
Includes Label or Dye	Yes
Reaction Format	SuperMix or Master Mix
Product Type	Direct PCR Master Mix
Sample Type	Tissue
Reaction Speed	Fast
Hot Start	Built-In Hot Start
Overhang	Blunt
Starting Material	DNA
For Use With (Application)	Genotyping
PCR Method	Direct PCR
Product Line	Phire

Thermo Scientific™ Phusion Human Specimen Direct PCR Kit

Thermo Scientific Phusion Human Specimen Direct PCR Kit delivers unprecedented convenience for DNA amplification by allowing PCR directly from unpurified human samples.

Pricing & Availability

Invitrogen™ Cells-to-cDNA™ II Kit

The Ambion™ Cells-to-cDNA™ II Kit (patent pending) produces cDNA from cultured mammalian cells in less than 2 hr., no RNA isolation is required.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
Content And Storage	1X PBS, Cell Lysis II Buffer, μL DNase 1, 10X RT Buffer, M-mLV Reverse Transcriptase, RNase Inhibitor, dNTP Mix, Random Decamers, Oligo(dT)₁₈ Primers, Armored RNA™ Control, Armored RNA™ Primer Pair, and Endogenous Primer Pair should be stored at -20°C. Nuclease-free water may be stored at any temperature.
Reverse Transcriptase	M-MLV
Size (Final Product)	7 kb or less
Final Product Type	cDNA
Sample Type	Mammalian Cells
Purification Time	2 hr.
Optimal Reaction Temperature	42°C
For Use With (Application)	Real Time PCR (qPCR),RT-PCR
Product Line	Ambion™, Cells-to-cDNA™

Thermo Scientific™ Phire Animal Tissue Direct PCR Kit (without sampling tools)

Thermo Scientific Phire Animal Tissue Direct PCR Kit has been developed for amplification of DNA directly from a wide variety of animal tissues including mice, fish, birds and insects.

Pricing & Availability

Thermo Scientific™ GeneJET PCR Purification Kit

Thermo Scientific GeneJET PCR Purification Kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions.

Pricing & Availability
Specifications

Format	Spin Column
Run Time	5 min.
High-throughput Compatibility	Not High-throughput Compatible (Manual)

Invitrogen™ Cells-to-CT™ 1-Step TaqMan™ Kit

Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step TaqMan™ Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.

Pricing & Availability
Specifications

GC-Rich PCR Performance	High
Format	Tube
Product Type	1-Step qPCR kit
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™, ViiA™ 7 System, StepOnePlus™ System, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, 7500 Fast Dx System, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Sample Type	Cell, RNA
Reaction Speed	Fast or Standard
Reagent Type	Cell Lysis
Green Features	Fewer resources used and less waste, Less hazardous
For Use With (Application)	Gene Expression
PCR Method	1-step RT-qPCR
Product Line	Cells-to-CT™, TaqMan™
Detection Method	Primer-probe

Invitrogen™ TaqMan™ Fast Advanced Cells-to-CT™ Kit

The TaqMan Fast Advanced Cells-to-Ct Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	2X
Green Features	Fewer resources used, less waste, less hazardous
Concentration	2X
For Use With (Application)	Gene Expression
High-throughput Compatibility	High-throughput Compatible
PCR Method	2-step RT-qPCR
Detection Method	TaqMan
Format	Tube
Product Type	Fast Advanced Cells-to-Ct Kit
Sample Type	Mammalian cells
Reaction Speed	7 min.
Reagent Type	Cell Lysis
Purification Target	Lysate
Starting Material	10 to 10,000 cells
Product Line	Cells-to-CT™

Thermo Scientific™ Phire Plant Direct PCR Master Mix PROMO

Amplify DNA directly from plant samples without prior DNA purification with Phire Plant Direct PCR Master Mix.

Promotions are available

Pricing & Availability
Specifications

Polymerase	Phire Hot Start II DNA polymerase
GC-Rich PCR Performance	Medium
Reaction Format	SuperMix or Master Mix
Product Type	Direct PCR Master Mix
Sample Type	Plant
Reaction Speed	Fast
Hot Start	Built-In Hot Start
Overhang	Blunt
Starting Material	DNA
For Use With (Application)	Genotyping
PCR Method	Direct PCR
Product Line	Phire

Thermo Scientific™ Phire Tissue Direct PCR Master Mix

Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects.

Pricing & Availability
Specifications

Shipping Condition	Wet or Dry Ice
GC-Rich PCR Performance	Medium
Includes Label or Dye	Yes
Overhang	Blunt
Concentration	2X
For Use With (Application)	Genotyping,Hot-start PCR
PCR Method	Direct PCR
Polymerase	Phire Hot Start II DNA Polymerase
Reaction Format	SuperMix or Master Mix
Product Type	Direct PCR Master Mix
Sample Type	Tissue
Reaction Speed	Fast
Hot Start	Built-In Hot Start
Starting Material	DNA

Invitrogen™ TaqMan™ Gene Expression Cells-to-C_T™ Kit

The TaqMan™ Gene Expression Cells-to-C_T™ Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Purity or Quality Grade	UP (Ultra Pure)
Reverse Transcriptase	M-MLV
Green Features	Fewer resources used and less waste, Less hazardous
PCR Method	2-step RT-qPCR
Detection Method	Primer-probe
Polymerase	DNA Polymerase
Format	Tube
Product Type	Gene Expression Cells-to-C Kit
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7000 System, 7300 System, 7500 System, 7900HT System, Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Sample Type	Cell
Reaction Speed	Standard
Product Line	Ambion™, Cells-to-CT™, TaqMan™

Direct PCR Reagents and Kits

Direct PCR Reagents and Kits

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