Transcription and Translation Reagents

A variety of kits, systems, buffers, inhibitors, RNA cap analogs, reagents, and solutions for use in experiments involving transcription and translation. Products can be used in the synthesis of RNA probes.

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Invitrogen™ MEGAscript™ T7 Transcription Kit

Ultra-high yield technology for in vitro transcription

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Invitrogen™ mMESSAGE mMACHINE™ T7 Transcription Kit

in vitro synthesis of large amounts of capped RNA

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Invitrogen™ ARCA (Anti-Reverse Cap Analog)

Cap analog for in vitro transcription for capped transcripts generation

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Invitrogen™ mMESSAGE mMACHINE™ T7 ULTRA Transcription Kit

Generate RNA transcripts with higher protein yields

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Invitrogen™ MEGAclear™ Transcription Clean-Up Kit

Quickly cleanup large-scale transcription reactions

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Invitrogen™ Cap Analog (m⁷G(5')ppp(5')G), 100 A₂₅₄ Units

For 5' capped RNA molecules synthesis as part of in vitro transcription reactions

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Promega RNasin™ Ribonuclease Inhibitor: Natural

Ribonuclease inhibitors with broad-spectrum RNase inhibitory properties. Recombinant and native available. Plus version protects RNA before, during and after heating.

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Specifications

Formulation	Amount of RNasin™ RNase inhibitor required to inhibit the activity of 5ng of RNase A by 50%, activity is measured by the inhibition of hydrolysis of Cytidine 2,3'-cyclic Monophosphate by RNase A
Concentration	20 to 40 U/μL
For Use With (Application)	RT-PCR, protection of mRNA in cDNA synthesis reactions, in-vitro transcription/translation, Increase yield and activity of polysomes, improvement of in-vitro virus replication, improvement of RNA translation in homologous systems, performing RNase-free Antibody staining, enhancement of RNA yields from Riboprobe™ system RNA synthesis reactions

Invitrogen™ mMESSAGE mMACHINE™ SP6 Transcription Kit

in vitro synthesis of large amounts of capped RNA

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Invitrogen™ CytoTune™-iPS 2.0 Sendai Reprogramming Kit

The CytoTune™-iPS 2.0 Sendai Reprogramming Kit improves upon the well-referenced CytoTune™-iPS Sendai Reprogramming Kit.

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Specifications

Shipping Condition	Dry Ice
Promoter	Proprietary
Format	Kit
Sub Cellular Localization	Cytoplasm
Product Type	Sendai Reprogramming Kit
Delivery Type	Sendai virus
For Use With (Application)	Stem Cell Engineering & Reprogramming
Product Line	CytoTune™

Invitrogen™ mMESSAGE mMACHINE™ T3 Transcription Kit

Obtain 10–50 times yield of conventional in vitro transcription reactions

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Invitrogen™ MEGAshortscript™ T7 Transcription Kit

Maximize the yield of short RNA transcripts

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Invitrogen™ MAXIscript™ T7 Transcription Kit

Fast synthesis of RNA probes for use in ribonuclease protection assays, in situ hybridization, and blot hybridizations

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Specifications

Promoter	T7
Content And Storage	T7 Enzyme Mix, 10X Transcription Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, TURBO DNase, Gel Loading Buffer II, and pTRI-b-actin are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
Format	Kit
Product Type	T7 In Vitro Transcription kit
Final Product Type	Synthetic mRNA,mRNA
Sample Type	mRNA,Synthetic mRNA
Yield	2-6 μg
Starting Material	PCR Products, Linearized DNA plasmid
For Use With (Application)	In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
Product Line	Ambion™, MAXIscript™

Invitrogen™ Retic Lysate IVT™ Kit

Efficiently translates high molecular weight proteins and both capped and uncapped mRNAs

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Specifications

No. of Reactions	60 Reactions
Expression Mechanism	Cell-Free Expression
Format	Kit
Product Type	Retic Lysate
Expressed Protein Type	Water Soluble Protein
Quantity	60 Reactions
For Use With (Application)	In Vitro Transcription
Expression System	Cell-Free
Product Line	Ambion™

Invitrogen™ Lipofectamine™ 2000 Transfection Reagent

Versatile transfection reagent that has been shown to effectively transfect the widest variety of adherent and suspension cell lines

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Invitrogen™ Cap Analog (m⁷G(5')ppp(5')G), 10 A₂₅₄ units

For 5' capped RNA molecules synthesis as part of in vitro transcription reactions

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Transcription and Translation Reagents

Transcription and Translation Reagents

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